Description
2 × EpiArtTM HS Taq Master Mix is suitable for amplifying bisulfite-converted DNA, and contains EpiArtTM HS Taq DNA Polymerase, dNTP and an
optimized buffer. Compared with common DNA, bisulfite conversion results in severe DNA damage and affects PCR performance. This product uses
the enzyme-engineered Hot-Start EpiArtTM HS Taq DNA Polymerase and an optimized-buffer system to achieve superior amplification efficiency,
sensitivity and specificity for bisulfite-converted DNA compared with common PCR reagents.
The pre-formulated 2 × Master Mix can be used for PCR reactions by simply adding primers and templates to reduce pipetting and improve throughput
and the reproducibility of results. EM202 contains a blue dye that can be directly electrophoresed after the reaction is completed, which is convenient
to use.
This product can be used with the EpiArtTM DNA Methylation Bisulfite Kit (Vazyme #EM101). The PCR products contain A at 3′-end, whichcan be
directly cloned into the T vector and applied to ClonExpress® Rapid Cloning Kit (Vazyme #C112/C113/C115) and 5 min TA/Blunt-Zero Cloning Kit
(Vazyme #C601).