2019-Novel Coronavirus (2019-nCoV) Triplex RT-qPCR Detection Kit

qPCR Kit for Real-time PCR instruments with FAM, TEXAS RED/ROX and HEX/VIC channels, such as ABI7500, ABI Q3, ABI Q6, Roche LightCycler480, Bio-Rad CFX96.

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Description

PRINCIPLE OF DETECTION

This product provides triplex-detections in a single tube, including two independent genes of 2019-nCoV and an internal control which targets the human RNAse P (RNP) gene to assess specimen quality. Specific primers and probes were designed for the detection of conserved region of 2019-nCoV’s ORF1ab gene (RdRP region) and N gene, respectively, avoiding non-specific interference of SARS2003 and BatSARS-like virus strains. Internal control (RNAse P gene) provides a nucleic acid extraction procedural control and a secondary negative control. Positive control (2019-nCoV-pseudoviruse) provides a nucleic acid extraction and a reverse transcription control to validate the entire procedure and reagent integrity.

PRODUCT CONTENTS

ComponentsAmountIngredientCap Color
Detection Buffer900 μl × 3 TubesBuffer, dNTPs, Primers, Probes.
Enzyme Mix400 μl × 1 TubeRNase Inhibitor, UDG, Reverse Transcriptase, Taq DNA polymerase.
Positive Control250 μl × 1 TubeRNA pseudovirus containing target gene.
Negative Control250 μl × 1 TubeDEPC-Treated Water.

SAMPLING & HANDLING

  • Suitable specimen type: upper respiratory specimen (including nasal swabs, nasopharyngeal swabs / aspirates / washes, and sputum) and lower respiratory specimen (including respiratory aspirates, bronchial washes, bronchoalveolar lavage fluids, and lung biopsy specimens).
  • For detailed methods of specimen collection, please refer to the protocol in the “Microbiology Specimen Collection Manual”.
  • The collected specimen should be used for detection within the same day. Otherwise, please store the specimen as follows:
    – Store at 2°C – 8°C for no more than 24 hours;
    – Store at < -20°C for no more than 10 days;
    – Store at < -70°C for long-term, avoiding repeated freeze-thaw cycles.
  • The specimen should be transported using sealed foam box with dry ice.

Additional information

Brand

Vazyme Biotech

Size

100 Tests

Storage

Store at -20°C

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