The ClonExpress cloning technology is simple, fast, and high efficient which enables directional insertion of any amplified DNA product into any
linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is
added onto the insert through PCR. The insert and the linearized vector, with overlapped sequences of 15 bp – 20 bp on both 5’- and 3’-end,
respectively, are mixed in an appropriate ratio and incubated with recombinase Exnase at 50℃ for 5 – 15 min.
The Vazyme ClonExpress UItra One Step Cloning kit is a novel cloning kit, applicable for homologous recombination with 1 – 5 fragments.
The Kit is independent of DNA ligase, significantly reducing the self-ligated colonies. The enhanced Exnase and highly optimized buffer
included in 2X ClonExpress Mix significantly improve the recombination efficiency and the tolerance to impurities. pCE-Zero vector is compatible
with most PCR products, enabling the specific PCR products to be used directly for recombination without any treatments, which significantly
simplify the procedure.
ClonExpress Ultra One Step Cloning KitClonExpress Ultra One Step Cloning Kit