The ExFect2000 Transfection Reagent is a novel and efficient transfection reagent based on cationic liposomes, which is suitable for DNA transfection and co-transfection systems for most eukaryotic cells (both adherent and suspended). The ExFect2000 has a unique structure and an optimized formulation. The presence of serum and antibiotics will not affect the efficiency of transfection, thereby reducing the effect of serum-deprival on cells. The ExFect2000 is also with low cytotoxicity. After transfection, there’s no need to remove nucleic acid- ExFect2000 complex or replace medium within 24 hr-48 hr. The high transfection efficiency of ExFect2000 transfection reagent has been validated in a board range of eukaryotic cell lines.