{"product_id":"2-phanta-ultra-master-mix","title":"2 × Phanta Ultra Master Mix","description":"\u003cp\u003e\nThe \u003cstrong\u003e2 × Phanta Ultra Master Mix\u003c\/strong\u003e from \u003cstrong\u003eVazyme\u003c\/strong\u003e is a ready-to-use high-fidelity PCR master mix designed for fast, accurate and robust DNA amplification. It is based on Phanta Ultra DNA Polymerase and is suitable for demanding molecular biology workflows such as molecular cloning, gene identification, gene synthesis and site-directed mutagenesis.\n\u003c\/p\u003e\n\n\u003ch3\u003eProduct overview\u003c\/h3\u003e\n\u003cp\u003e\nThis 2× master mix contains the essential PCR components required for amplification, including Phanta Ultra DNA Polymerase, dNTPs, Mg\u003csup\u003e2+\u003c\/sup\u003e and an optimized reaction buffer. Only primers, template DNA and nuclease-free water need to be added, helping to reduce pipetting steps and improve reproducibility.\n\u003c\/p\u003e\n\n\u003ch3\u003eKey features\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eHigh-fidelity PCR master mix for accurate DNA amplification\u003c\/li\u003e\n  \u003cli\u003eApproximately 200× higher fidelity than standard Taq DNA polymerase\u003c\/li\u003e\n  \u003cli\u003eFast extension speed: 5 sec\/kb for fragments below 10 kb and 10 sec\/kb for fragments of 10 kb or longer\u003c\/li\u003e\n  \u003cli\u003eUniversal annealing program with primer annealing at 60°C\u003c\/li\u003e\n  \u003cli\u003eBroad template compatibility, including complex templates and GC-rich or AT-rich systems\u003c\/li\u003e\n  \u003cli\u003eSuitable for multiplex amplification workflows\u003c\/li\u003e\n  \u003cli\u003eCan amplify long fragments up to 40 kb, depending on template quality and reaction conditions\u003c\/li\u003e\n  \u003cli\u003eProduces blunt-ended PCR products suitable for downstream cloning workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eHigh-fidelity PCR\u003c\/li\u003e\n  \u003cli\u003eMolecular cloning\u003c\/li\u003e\n  \u003cli\u003eGene identification\u003c\/li\u003e\n  \u003cli\u003eGene synthesis\u003c\/li\u003e\n  \u003cli\u003eSite-directed mutagenesis\u003c\/li\u003e\n  \u003cli\u003eLong-fragment PCR\u003c\/li\u003e\n  \u003cli\u003eMultiplex PCR\u003c\/li\u003e\n  \u003cli\u003eAmplification of GC-rich, AT-rich or complex DNA templates\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Vazyme Biotech","offers":[{"title":"1 ml","offer_id":58144258097497,"sku":"P518-01","price":95.0,"currency_code":"EUR","in_stock":true},{"title":"5 × 1 ml","offer_id":58144258130265,"sku":"P518-02","price":304.0,"currency_code":"EUR","in_stock":true},{"title":"15 × 1 ml","offer_id":58144258163033,"sku":"P518-03","price":827.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/2x-phanta-ultra-master-mix.png?v=1779828084","url":"https:\/\/absource.de\/products\/2-phanta-ultra-master-mix","provider":"Absource Diagnostics","version":"1.0","type":"link"}