{"product_id":"as160-antibody-sc-f0418","title":"AS160 Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eAS160, also known as TBC1D4, is a 160 kDa substrate of the Akt Ser\/Thr kinase, functioning as a Rab GTPase-activating protein pivotal in regulating insulin-induced Glut4 trafficking. Its expression spans various tissues, including brain, kidney, liver, and both brown and white fat. Upon insulin stimulation, AS160 undergoes phosphorylation at multiple Akt phosphorylation sites, particularly at Ser318, Ser570, Ser588, Thr642, and Thr751, enhancing its activity. Depending on the literature source, AS160 may also be discussed as AS160 (Rab-GAP).\u003c\/p\u003e\u003cp\u003eReported cellular context includes cytoplasm, which can matter when signal is compared across treatments or changing cell states. Following AS160 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eAS160 is commonly interpreted in the context of cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003esignal enrichment within cytoplasm relative to the broader cellular background\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003cli\u003etime-matched comparisons so changes reflect biology rather than handling or sampling drift\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for AS160. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in AS160 reflect biology rather than handling. When interpreting AS160, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep AS160 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577451356505,"sku":"F0418-20UL","price":149.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577451389273,"sku":"F0418-100UL","price":329.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577451422041,"sku":"F0418-2X100UL","price":489.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F0418-wb.gif?v=1773598443","url":"https:\/\/absource.de\/products\/as160-antibody-sc-f0418","provider":"Absource Diagnostics","version":"1.0","type":"link"}