{"product_id":"cd3z-antibody-sc-f2899","title":"Phospho-CD3 ζ (Tyr83) Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eCD3Z is a target of interest in many antibody-based workflows. Phospho-CD3 ζ (Tyr83) refers to the phosphorylation of tyrosine 83 within one of the immunoreceptor tyrosine-based activation motifs (ITAMs) of the CD3 zeta chain, a critical component of the T cell receptor (TCR) complex. Upon TCR engagement by antigen-presenting cells, Src family kinases such as Lck and Fyn phosphorylate Tyr83, along with other ITAM tyrosines, creating docking sites for SH2 domain-containing signaling proteins, most notably ZAP-70. Depending on the literature source, CD3Z may also be discussed as Phospho-CD3 zeta (Tyr83) and CD247.\u003c\/p\u003e\u003cp\u003eReported cellular context includes cellmembrane and membrane, which can matter when signal is compared across treatments or changing cell states. Following CD3Z across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eCD3Z is commonly interpreted in the context of immunology and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cellmembrane and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cellmembrane and membrane across matched conditions\u003c\/li\u003e\n\u003cli\u003econtext differences tied to immune-cell state, activation, or lineage composition\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003cli\u003edifferences between total target abundance and site-specific regulation when modified forms are compared\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for CD3Z. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in CD3Z reflect biology rather than handling. When interpreting CD3Z, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep CD3Z trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577984295257,"sku":"F2899-20UL","price":199.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577984328025,"sku":"F2899-100UL","price":489.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577984360793,"sku":"F2899-2X100UL","price":729.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F2899-IF.png?v=1773600791","url":"https:\/\/absource.de\/products\/cd3z-antibody-sc-f2899","provider":"Absource Diagnostics","version":"1.0","type":"link"}