{"product_id":"discover-sc-wta-kit-v2","title":"Discover-sc WTA Kit V2","description":"\u003cp\u003e\nThe \u003cstrong\u003eDiscover-sc WTA Kit V2\u003c\/strong\u003e from \u003cstrong\u003eVazyme\u003c\/strong\u003e is designed for whole transcriptome amplification from single cells and other low-input RNA samples. The kit enables first-strand cDNA synthesis and amplification from very small starting amounts, helping researchers generate sufficient cDNA for downstream sequencing and transcriptome analysis workflows.\n\u003c\/p\u003e\n\n\u003ch3\u003eProduct overview\u003c\/h3\u003e\n\u003cp\u003e\nDiscover-sc WTA Kit V2 uses \u003cstrong\u003eOligo dT priming\u003c\/strong\u003e for reverse transcription and the template-switching activity of \u003cstrong\u003eDiscover-sc Reverse Transcriptase\u003c\/strong\u003e to add an adapter sequence during cDNA synthesis. The resulting cDNA is then amplified by PCR to generate full-length cDNA amplification products. This workflow helps reduce 3' bias and rRNA contamination while supporting full transcriptome coverage.\n\u003c\/p\u003e\n\n\u003ch3\u003eKey features\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eDesigned for single-cell and low-input whole transcriptome amplification\u003c\/li\u003e\n  \u003cli\u003eSuitable for 1–1,000 cells or 10 pg–10 ng total RNA input\u003c\/li\u003e\n  \u003cli\u003eUses Oligo dT priming for poly(A)+ RNA-derived cDNA synthesis\u003c\/li\u003e\n  \u003cli\u003eTemplate-switching reverse transcription enables full-length cDNA amplification\u003c\/li\u003e\n  \u003cli\u003eImproved sensitivity for low-abundance genes and low-concentration templates\u003c\/li\u003e\n  \u003cli\u003eHelps reduce 3' end preference and rRNA contamination during cDNA synthesis\u003c\/li\u003e\n  \u003cli\u003eAmplified cDNA can be used for downstream sequencing library preparation workflows\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n  \u003cli\u003eSingle-cell transcriptome sequencing workflows\u003c\/li\u003e\n  \u003cli\u003eWhole transcriptome amplification\u003c\/li\u003e\n  \u003cli\u003eLow-input RNA sequencing sample preparation\u003c\/li\u003e\n  \u003cli\u003eGene expression analysis\u003c\/li\u003e\n  \u003cli\u003eFusion transcript analysis\u003c\/li\u003e\n  \u003cli\u003eAlternative splicing analysis\u003c\/li\u003e\n  \u003cli\u003eFull-length cDNA amplification\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Vazyme Biotech","offers":[{"title":"12 RXNS","offer_id":58143936315737,"sku":"N711-01","price":869.0,"currency_code":"EUR","in_stock":true},{"title":"24 RXNS","offer_id":58143936348505,"sku":"N711-02","price":1487.0,"currency_code":"EUR","in_stock":true},{"title":"96 RXNS","offer_id":58143936381273,"sku":"N711-03","price":4285.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/discover-sc-wta-kit-v2.png?v=1779824376","url":"https:\/\/absource.de\/products\/discover-sc-wta-kit-v2","provider":"Absource Diagnostics","version":"1.0","type":"link"}