{"product_id":"elisa-coating-buffer","title":"ELISA Coating Buffer","description":"\u003cp\u003eELISA Coating Buffer is a sterile-filtered 1x carbonate\/bicarbonate buffer designed for coating ELISA microplates with antibodies or antigens. During the plate coating step, the buffer provides salt and pH conditions that support adsorption of proteins to the microplate surface while helping maintain protein solubility and conformational integrity.\u003c\/p\u003e \u003ch3\u003eKey features\u003c\/h3\u003e \u003cul\u003e \u003cli\u003eSterile-filtered 1x carbonate\/bicarbonate coating buffer\u003c\/li\u003e \u003cli\u003eDesigned for ELISA microplate coating workflows\u003c\/li\u003e \u003cli\u003eSupports immobilization of antibodies or antigens onto microplate surfaces\u003c\/li\u003e \u003cli\u003eHelps maintain protein solubility and conformational integrity during coating\u003c\/li\u003e \u003cli\u003ePreservative-free formulation\u003c\/li\u003e \u003cli\u003eAvailable in 50 ml, 100 ml and 250 ml sizes\u003c\/li\u003e \u003c\/ul\u003e \u003ch3\u003eApplications\u003c\/h3\u003e \u003cul\u003e \u003cli\u003eELISA plate coating\u003c\/li\u003e \u003cli\u003eAntibody immobilization on high-bind microplates\u003c\/li\u003e \u003cli\u003eAntigen immobilization on ELISA plate surfaces\u003c\/li\u003e \u003cli\u003eResearch-use immunoassay development workflows\u003c\/li\u003e \u003c\/ul\u003e \u003ch3\u003eUse in ELISA workflows\u003c\/h3\u003e \u003cp\u003eDilute the antibody or antigen into the coating buffer at an empirically optimized coating concentration. Mix the solution and add 50–200 µl to each well of the microplate, preferably using a high-bind polystyrene microtiter plate. Incubate the coating solution for 8–24 hours at room temperature protected from light, while minimizing evaporation with an adhesive plate sealer.\u003c\/p\u003e \u003cp\u003eAfter coating, aspirate the coating solution, wash the wells 2–3 times with 1x ELISA Wash Buffer, and continue with the blocking step using an appropriate ELISA blocking or stabilizing solution.\u003c\/p\u003e","brand":"Cytodiagnostics","offers":[{"title":"50 ml","offer_id":58159681765721,"sku":"EL-C9-50ML","price":50.0,"currency_code":"EUR","in_stock":true},{"title":"100 ml","offer_id":58159681798489,"sku":"EL-C9-100ML","price":85.0,"currency_code":"EUR","in_stock":true},{"title":"250 ml","offer_id":58159681831257,"sku":"EL-C9-250ML","price":165.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/elisa-coating-buffer_png.webp?v=1779967653","url":"https:\/\/absource.de\/products\/elisa-coating-buffer","provider":"Absource Diagnostics","version":"1.0","type":"link"}