{"product_id":"elisa-detection-diluent","title":"ELISA Detection Diluent","description":"\u003cp\u003eELISA Detection Diluent is a ready-to-use 1x buffered solution for diluting detection primary antibodies and HRP conjugates in ELISA workflows. It is formulated with mammalian protein, detergent and salt additives to support antibody and HRP-conjugate stability while helping reduce non-specific background binding.\u003c\/p\u003e \u003ch3\u003eKey features\u003c\/h3\u003e \u003cul\u003e \u003cli\u003eReady-to-use 1x ELISA detection diluent\u003c\/li\u003e \u003cli\u003eDesigned for dilution of detection antibodies and HRP conjugates\u003c\/li\u003e \u003cli\u003eContains mammalian protein, detergent and salt additives\u003c\/li\u003e \u003cli\u003eHelps reduce non-specific binding to sample proteins, capture antibody and ELISA plate surfaces\u003c\/li\u003e \u003cli\u003eAseptically filtered through a 0.2 µm filter\u003c\/li\u003e \u003cli\u003eContains a non-azide, non-mercury preservative\u003c\/li\u003e \u003cli\u003eAvailable in 50 ml, 100 ml and 250 ml sizes\u003c\/li\u003e \u003c\/ul\u003e \u003ch3\u003eApplications\u003c\/h3\u003e \u003cul\u003e \u003cli\u003eDilution of detection primary antibodies\u003c\/li\u003e \u003cli\u003eDilution of secondary antibody-HRP conjugates\u003c\/li\u003e \u003cli\u003eDilution of streptavidin-HRP conjugates when using biotinylated detection antibodies\u003c\/li\u003e \u003cli\u003eELISA detection workflows\u003c\/li\u003e \u003cli\u003eResearch-use immunoassay assay development\u003c\/li\u003e \u003c\/ul\u003e \u003ch3\u003eUse in ELISA workflows\u003c\/h3\u003e \u003cp\u003eThe diluent can be used to prepare detection primary antibody solutions and HRP-conjugate solutions at the desired working concentration. Cytodiagnostics states that detection antibody concentrations typically range from 0.05–0.5 µg\/ml, depending on the ELISA protocol and antibody used.\u003c\/p\u003e","brand":"Cytodiagnostics","offers":[{"title":"50 ml","offer_id":58159558984025,"sku":"EL-C4-50ML","price":50.0,"currency_code":"EUR","in_stock":true},{"title":"100 ml","offer_id":58159559016793,"sku":"EL-C4-100ML","price":85.0,"currency_code":"EUR","in_stock":true},{"title":"250 ml","offer_id":58159559049561,"sku":"EL-C4-250ML","price":165.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/elisa-detection-diluent_png.webp?v=1779966154","url":"https:\/\/absource.de\/products\/elisa-detection-diluent","provider":"Absource Diagnostics","version":"1.0","type":"link"}