{"product_id":"elisa-stop-solution","title":"ELISA Stop Solution","description":"\u003cp\u003eELISA Stop Solution is a ready-to-use acidic solution for terminating the TMB substrate-peroxidase reaction in endpoint colorimetric ELISA assays. After stopping the reaction, the TMB chromogen changes from blue to yellow, enabling absorbance measurement at 450 nm.\u003c\/p\u003e \u003ch3\u003eKey features\u003c\/h3\u003e \u003cul\u003e \u003cli\u003eReady-to-use acidic ELISA stop solution\u003c\/li\u003e \u003cli\u003eDesigned for endpoint ELISA assays using TMB substrate\u003c\/li\u003e \u003cli\u003eStops the TMB substrate-peroxidase reaction\u003c\/li\u003e \u003cli\u003eChanges the reaction color from blue to yellow after stopping\u003c\/li\u003e \u003cli\u003eSupports absorbance measurement at 450 nm\u003c\/li\u003e \u003cli\u003eAvailable in 50 ml, 100 ml and 250 ml sizes\u003c\/li\u003e \u003c\/ul\u003e \u003ch3\u003eApplications\u003c\/h3\u003e \u003cul\u003e \u003cli\u003eEndpoint colorimetric ELISA assays\u003c\/li\u003e \u003cli\u003eStopping TMB substrate reactions\u003c\/li\u003e \u003cli\u003eHRP\/peroxidase-based ELISA detection workflows\u003c\/li\u003e \u003cli\u003eResearch-use immunoassay assay development\u003c\/li\u003e \u003c\/ul\u003e \u003ch3\u003eUse in ELISA workflows\u003c\/h3\u003e \u003cp\u003eEquilibrate the ELISA Stop Solution to room temperature before use. After the TMB substrate reaction has developed to the desired intensity, add 50–100 µl stop solution to each plate well. Read absorbance at 450 nm within 10 minutes. For best results, monitor sample absorbance and stop the reaction before values exceed 2.0 absorbance units.\u003c\/p\u003e","brand":"Cytodiagnostics","offers":[{"title":"50 ml","offer_id":58159599714649,"sku":"EL-C8-50ML","price":50.0,"currency_code":"EUR","in_stock":true},{"title":"100 ml","offer_id":58159599747417,"sku":"EL-C8-100ML","price":85.0,"currency_code":"EUR","in_stock":true},{"title":"250 ml","offer_id":58159599780185,"sku":"EL-C8-250ML","price":165.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/elisa-stop-solution_png.webp?v=1779966696","url":"https:\/\/absource.de\/products\/elisa-stop-solution","provider":"Absource Diagnostics","version":"1.0","type":"link"}