{"product_id":"mad3-antibody-sc-f0197","title":"Phospho-IκBα (Ser32\/36) Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eMAD3 is a target of interest in many antibody-based workflows. The IκB kinase (IKK) complex serves as a central signaling hub for the activation of the NF-κB pathway. This complex is composed of two catalytic subunits, IKKα and IKKβ, which are serine\/threonine kinases, and a regulatory subunit known as NEMO (NF-κB essential modulator), also referred to as IKKγ. Depending on the literature source, MAD3 may also be discussed as Phospho-IkappaBalpha (Ser32\/36) and NF-kappa-B inhibitor alpha.\u003c\/p\u003e\u003cp\u003eReported cellular context includes cytoplasm and nucleus, which can matter when signal is compared across treatments or changing cell states. Following MAD3 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eMAD3 is commonly interpreted in the context of immunology, inflammation, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm and nucleus, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cytoplasm and nucleus across matched conditions\u003c\/li\u003e\n\u003cli\u003econtext differences tied to immune-cell state, activation, or lineage composition\u003c\/li\u003e\n\u003cli\u003eresponses associated with cytokine exposure, inflammatory tone, or tissue stress\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for MAD3. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in MAD3 reflect biology rather than handling. When interpreting MAD3, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep MAD3 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577424191833,"sku":"F0197-20UL","price":189.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577424224601,"sku":"F0197-100UL","price":419.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577424257369,"sku":"F0197-2X100UL","price":629.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F0197-wb.gif?v=1773598192","url":"https:\/\/absource.de\/products\/mad3-antibody-sc-f0197","provider":"Absource Diagnostics","version":"1.0","type":"link"}