{"product_id":"pras40-antibody-sc-f4154","title":"Phospho-PRAS40 (Thr246) Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003ePRAS40 (proline-rich AKT substrate of 40 kDa, encoded by AKT1S1) functions as a negative regulator of the mammalian target of rapamycin complex 1 (mTORC1). It exerts this effect by binding to Raptor and competing with mTOR substrates such as 4E-BP1 and p70S6K. Both PKB\/AKT and mTOR phosphorylate PRAS40 at distinct residues-Thr246 by AKT, and Ser183\/212\/221 by mTOR-in vitro and in vivo. Depending on the literature source, PRAS40 may also be discussed as Phospho-PRAS40 (Thr246) and Proline-rich AKT1 substrate 1.\u003c\/p\u003e\u003cp\u003eReported cellular context includes cytoplasm, which can matter when signal is compared across treatments or changing cell states. Following PRAS40 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003ePRAS40 is commonly interpreted in the context of cancer, stem cell biology, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003esignal enrichment within cytoplasm relative to the broader cellular background\u003c\/li\u003e\n\u003cli\u003echanges associated with proliferative state, oncogenic signaling, or treatment response\u003c\/li\u003e\n\u003cli\u003estate transitions between self-renewal, priming, and differentiation\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for PRAS40. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in PRAS40 reflect biology rather than handling. When interpreting PRAS40, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep PRAS40 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57578066116953,"sku":"F4154-20UL","price":189.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57578066149721,"sku":"F4154-100UL","price":459.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57578066182489,"sku":"F4154-2X100UL","price":679.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F4154-wb.gif?v=1773601661","url":"https:\/\/absource.de\/products\/pras40-antibody-sc-f4154","provider":"Absource Diagnostics","version":"1.0","type":"link"}