{"product_id":"vcl-antibody-sc-f0110","title":"Vinculin Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eVinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. Vinculin comprises three major domains- an N-terminal head, a flexible proline-rich hinge (neck) region, and a C-terminal tail domain. Vinculin activation results from conformational rearrangements of these domains. Depending on the literature source, VCL may also be discussed as Vinculin.\u003c\/p\u003e\u003cp\u003eReported cellular context includes cell membrane, cell junction, cytoplasm, and cytoskeleton, which can matter when signal is compared across treatments or changing cell states. Following VCL across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eVCL is commonly interpreted in the context of cancer and developmental biology research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell membrane, cell junction, and cytoplasm, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cell membrane, cell junction, and cytoplasm across matched conditions\u003c\/li\u003e\n\u003cli\u003echanges associated with proliferative state, oncogenic signaling, or treatment response\u003c\/li\u003e\n\u003cli\u003estage-dependent patterns during differentiation, morphogenesis, or lineage commitment\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for VCL. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in VCL reflect biology rather than handling. When interpreting VCL, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep VCL trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577383919961,"sku":"F0110-20UL","price":169.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577383952729,"sku":"F0110-100UL","price":379.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577383985497,"sku":"F0110-2X100UL","price":569.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F0110-wb.gif?v=1773598127","url":"https:\/\/absource.de\/products\/vcl-antibody-sc-f0110","provider":"Absource Diagnostics","version":"1.0","type":"link"}