{"product_id":"vcl-antibody-sc-f3520","title":"Vinculin Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eVCL is a target of interest in many antibody-based workflows. Vinculin is a 117 kDa cytoskeletal protein that localizes to sites of integrin-dependent cell-matrix adhesions and cadherin-mediated cell-cell junctions. Structurally, vinculin consists of distinct head, neck, and tail domains. In its inactive form, the head and tail regions interact, concealing binding sites for various vinculin-associated proteins and maintaining a closed conformation. Depending on the literature source, VCL may also be discussed as Vinculin and Metavinculin (MV).\u003c\/p\u003e\u003cp\u003eReported cellular context includes cell junction, cell membrane, cell projection, and cytoplasm, which can matter when signal is compared across treatments or changing cell states. Following VCL across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eVCL is commonly interpreted in the context of cancer, developmental biology, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell junction, cell membrane, and cell projection, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cell junction, cell membrane, and cell projection across matched conditions\u003c\/li\u003e\n\u003cli\u003echanges associated with proliferative state, oncogenic signaling, or treatment response\u003c\/li\u003e\n\u003cli\u003estage-dependent patterns during differentiation, morphogenesis, or lineage commitment\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for VCL. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in VCL reflect biology rather than handling. When interpreting VCL, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep VCL trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57578028695897,"sku":"F3520-20UL","price":169.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57578028728665,"sku":"F3520-100UL","price":379.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57578028761433,"sku":"F3520-2X100UL","price":569.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F3520-wb.gif?v=1773601281","url":"https:\/\/absource.de\/products\/vcl-antibody-sc-f3520","provider":"Absource Diagnostics","version":"1.0","type":"link"}