2 × Phanta Ultra Master Mix (Dye Plus)

The 2× Phanta® Ultra Master Mix (Dye Plus) from Vazyme is a ready-to-use high-fidelity PCR master mix designed for fast, accurate, and reliable DNA amplification. Powered by Phanta Ultra DNA Polymerase, it delivers ultra-high fidelity, rapid extension, and robust performance across a wide range of templates.

The mix contains essential PCR components, including polymerase, dNTPs, Mg²⁺, reaction buffer, and loading dye. PCR products can be loaded directly onto agarose gels without adding separate loading buffer.

Key Features

• Ultra-high fidelity, approximately 200× higher than standard Taq polymerase
• Fast amplification with extension speeds of up to 5 sec/kb
• Optimized universal annealing temperature of 60°C
• Suitable for GC-rich, AT-rich, complex, and crude samples
• Supports long-fragment amplification up to 40 kb
• Hot-start technology for improved specificity
• Integrated loading dye for direct gel electrophoresis
• Produces blunt-end PCR products suitable for cloning workflows

Applications

• High-fidelity DNA amplification
• Molecular cloning
• DNA sequencing preparation
• Site-directed mutagenesis
• Long-range PCR
• Multiplex PCR
• Amplification of difficult templates

The 2× Phanta® Ultra Master Mix (Dye Plus) is ideal for demanding molecular biology workflows where speed, accuracy, and reproducibility are essential.