Catalog-Number:
P515
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Enhance your PCR results with 2 × Phanta Max Master Mix, designed for high-fidelity and rapid amplification. Ideal for various PCR applications, ensuring accuracy and efficiency.
Catalog-Number:
P518
Brand:
Nanjing Vazyme Biotech Co., Ltd.
The 2× Phanta Ultra Master Mix from Vazyme is a next-generation high-fidelity PCR master mix developed for ultra-accurate, rapid, and highly efficient DNA amplification. Powered by the advanced Phanta Ultra DNA Polymerase and optimized through the BioSmart platform, this master mix combines exceptional fidelity with fast extension rates and broad compatibility across diverse PCR applications.
The optimized hot-start system and superior reaction buffer provide outstanding amplification specificity and high success rates, even for challenging templates such as GC-rich, AT-rich, crude, or multiplex PCR systems. With a fidelity approximately 200× higher than conventional Taq polymerase, the kit is ideal for applications requiring highly precise amplification.
Key Features
Ultra-high fidelity: Approximately 200× higher fidelity than standard Taq DNA polymerase.
Rapid amplification: Extension speed of 5 sec/kb for fragments under 10 kb.
Universal annealing conditions: Broad Tm compatibility enables simplified PCR setup.
Hot-start technology: Improved specificity and reduced non-specific amplification.
Long fragment amplification: Supports amplification of fragments up to 40 kb.
Broad template compatibility: Suitable for genomic DNA, plasmids, cDNA, crude samples, GC-rich and AT-rich templates.
Ready-to-use format: Contains DNA polymerase, dNTPs, Mg2+, and optimized buffer.
Blunt-end PCR products: Compatible with downstream cloning workflows such as ClonExpress and TOPO cloning.
Applications
High-fidelity PCR amplification
Molecular cloning
DNA sequencing preparation
Site-directed mutagenesis
Long-range PCR
Multiplex PCR
GC-rich template amplification
Gene synthesis and verification
Advantages
The optimized reaction chemistry enables reliable amplification with minimal optimization, making the workflow highly efficient for routine and advanced molecular biology applications. The broad Tm compatibility allows multiple targets with varying primer melting temperatures to be amplified simultaneously using a unified PCR protocol.
Its combination of ultra-high fidelity, rapid cycling, and excellent robustness makes the 2× Phanta Ultra Master Mix an excellent choice for research applications where precision and speed are critical.
Catalog-Number:
P528
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
1-2 weeks
The 2× Phanta® Ultra Master Mix (Dye Plus) from Vazyme is a ready-to-use high-fidelity PCR master mix designed for fast, accurate, and reliable DNA amplification.
Powered by Phanta Ultra DNA Polymerase, it delivers ultra-high fidelity, rapid extension, and robust performance across a wide range of templates.
The mix contains essential PCR components, including polymerase, dNTPs, Mg2+, reaction buffer, and loading dye. PCR products can be loaded directly onto agarose gels without adding separate loading buffer.
Key Features
Ultra-high fidelity, approximately 200× higher than standard Taq polymerase
Fast amplification with extension speeds of up to 5 sec/kb
Optimized universal annealing temperature of 60°C
Suitable for GC-rich, AT-rich, complex, and crude samples
Supports long-fragment amplification up to 40 kb
Hot-start technology for improved specificity
Integrated loading dye for direct gel electrophoresis
Produces blunt-end PCR products suitable for cloning workflows
Applications
High-fidelity DNA amplification
Molecular cloning
DNA sequencing preparation
Site-directed mutagenesis
Long-range PCR
Multiplex PCR
Amplification of difficult templates
The 2× Phanta® Ultra Master Mix (Dye Plus) is ideal for demanding molecular biology workflows where speed, accuracy, and reproducibility are essential.
Catalog-Number:
P526
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Buy the 2× Phanta UniFi Master Mix (Dye Plus) by Vazyme – a high-fidelity, ready-to-use PCR mix with loading dye. Fast, accurate, and ideal for cloning, sequencing, and gel electrophoresis. Available at Absource Diagnostics
Catalog-Number:
P223
Brand:
Nanjing Vazyme Biotech Co., Ltd.
The 2× Rapid Taq Plus Master Mix (Dye Plus) from Vazyme is a high-performance ready-to-use PCR amplification solution designed for fast, reliable, and highly reproducible DNA amplification. The optimized premixed formulation contains Rapid Taq Plus DNA Polymerase, dNTPs, Mg2+, reaction buffer, stabilizers, and loading dye, significantly reducing pipetting steps and minimizing experimental variation.
Featuring advanced hot-start technology with dual-blocking antibodies and an optimized amplification system, this master mix delivers excellent specificity, sensitivity, and amplification success rates across a broad range of templates and GC contents.
Key Features
Ultra-fast PCR amplification: Extension speed as fast as 1–10 sec/kb, greatly reducing PCR runtime.
Ready-to-use formulation: Simply add primers and template DNA.
Hot-start technology: Dual-antibody blocking system improves specificity and sensitivity.
Broad template compatibility: Efficient amplification of genomic DNA, plasmid DNA, λDNA, and cDNA templates.
Supports long fragment amplification: Up to 15 kb genomic/plasmid targets and up to 10 kb cDNA targets.
Wide GC compatibility: Robust performance across low and high GC-content templates.
Direct gel loading: Integrated tracking dye allows direct electrophoresis after PCR.
Freeze-thaw stability: Special stabilizers prevent freezing at -20°C and maintain enzyme activity.
Applications
Rapid PCR amplification
Routine molecular cloning
Colony PCR
Genotyping
Template screening
DNA fragment amplification
High-throughput PCR workflows
Advantages
The optimized reaction chemistry provides high amplification efficiency and strong specificity while reducing overall workflow time. The included loading dye eliminates the need for additional sample preparation before agarose gel electrophoresis, streamlining daily PCR applications.
PCR products generated with this mix contain 3′-A overhangs, making them compatible with TA cloning workflows and Vazyme cloning systems such as ClonExpress and TOPO cloning kits.
Catalog-Number:
Q513
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Enhance your qPCR accuracy with AceQ Universal Probe Master Mix V2, designed for exceptional specificity and efficiency in probe-based assays, ideal for diagnostic and research applications.
Catalog-Number:
006805
Brand:
Bioenno Tech, LLC
Exp. delivery time:
1-2 weeks
Discover the Anti-freeze Cryoprotectant Solution (DEPC-treated), ideal for preserving fixed sections in research. Enhance your results with our reliable, RNA-safe formula, designed for flexibility and precision in scientific studies.
Catalog-Number:
003850
Brand:
Bioenno Tech, LLC
Exp. delivery time:
1-2 weeks
BDHC Substrate Kit: Enhanced Histochemical Staining
Unlock superior staining clarity with the BDHC Substrate Kit, your premier choice for histochemical analysis. Specially designed for researchers and laboratory professionals, this kit offers an advanced substrate solution for the detection of alkaline phosphatase activity in tissue sections, cell preparations, and substrate gels.
Exclusive Benefits
High Sensitivity: The BDHC substrate provides exceptional sensitivity, enabling the detection of minute enzyme activities for accurate and detailed analysis.
Vivid Color Development: Produces a distinct blue-black precipitate, ensuring clear and distinguishable staining results for both light and electron microscopy.
Wide Application: Versatile for use in various research fields, including immunohistochemistry, in situ hybridization, and zymography.
User Convenience: Ready-to-use formulation simplifies the staining process, reducing preparation time and enhancing workflow efficiency.
Kit Contents
BDHC Substrate Solution
Buffer Solution
Chromogen
Comprehensive User Guide
Optimal Applications
Designed for excellence in:
Immunohistochemistry (IHC)
In Situ Hybridization (ISH)
Enzyme Histochemistry
Zymography
Elevate your histochemical staining with the BDHC Substrate Kit, crafted for clarity, sensitivity, and ease of use in advanced research applications.
Catalog-Number:
Q711
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Enhance qPCR precision with ChamQ Universal SYBR qPCR Master Mix, offering unmatched efficiency and specificity for diverse applications, including gene expression and genotyping.
Catalog-Number:
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
1-2 weeks
ClonExpress Ultra One Step Cloning Kit V3 utilizes the latest optimized technology, enabling highly efficient homologous recombination of 1 - 11 DNA fragments. The newly optimized 2 × CE Mix V3 not only ensures a high cloning success rate for complicated situations such as large vectors and GC-rich/poor homologous arms, but also significantly enhances compatibility with amounts of DNA input. This greatly solves the problem of reduced cloning efficiency caused by low DNA fragment concentration.
Catalog-Number:
003836
Brand:
Bioenno Tech, LLC
Exp. delivery time:
1-2 weeks
DAB Substrate Kit: Precision Antigen Detection
Enhance your immunohistochemical staining with the DAB Substrate Kit, the gold standard for detecting horseradish peroxidase (HRP) activity in tissue sections and cell samples. Ideal for researchers and diagnosticians, this kit provides a reliable and sensitive method for visualizing antibody-antigen interactions, resulting in sharp, brown precipitates for clear microscopic analysis.
Key Features
High Sensitivity: Enables detection of low-abundance antigens, ensuring comprehensive immunohistochemical studies.
Crisp, Clear Staining: Produces distinct, brown precipitates for unambiguous identification of target antigens.
Wide Compatibility: Suitable for a variety of sample types, including formalin-fixed, paraffin-embedded (FFPE) tissues.
Easy to Use: Comes with a straightforward, user-friendly protocol, minimizing preparation time and effort.
Kit Contents
DAB Chromogen
Substrate Buffer
Stabilizer
Detailed Instruction Manual
Applications
The DAB Substrate Kit is essential for:
Diagnostic Immunohistochemistry
Research in Cellular and Molecular Pathology
Antibody Validation Studies
Tumor Marker Identification
With its superior sensitivity and ease of use, the DAB Substrate Kit is the preferred choice for professionals seeking precise antigen detection in IHC applications.
Catalog-Number:
003843
Brand:
Bioenno Tech, LLC
Exp. delivery time:
1-2 weeks
DAB-Co Substrate Kit: Intensified IHC Staining
Elevate your immunohistochemical analysis with the DAB-Co Substrate Kit. Engineered for researchers and diagnosticians, this kit incorporates cobalt enhancement for the DAB (3,3'-Diaminobenzidine) substrate, resulting in intensified, crisp, and dark blue-black staining of target antigens in tissue sections and cell samples.
Enhanced Features
Intensified Staining: Cobalt enhancement provides darker, more contrasted staining results, ideal for detailed morphological studies.
High Sensitivity and Specificity: Detects low-abundance proteins with high precision, reducing background noise and improving clarity.
Flexible Application: Compatible with a wide range of tissues, including FFPE samples, and suitable for both light and electron microscopy.
User-Friendly Protocol: Pre-mixed, ready-to-use solutions simplify the staining process, enhancing lab workflow and efficiency.
Kit Contents
DAB-Co Chromogen Solution
Substrate Buffer
Enhancer (Cobalt Solution)
Instruction Manual
Key Applications
The DAB-Co Substrate Kit is indispensable for:
Diagnostic Immunohistochemistry
Neuroscience Research
Cellular and Molecular Pathology
Biomarker Discovery
With its enhanced staining capabilities, the DAB-Co Substrate Kit sets a new standard for precision and clarity in IHC analysis.
Catalog-Number:
DD3509EN
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Streamline RNA research with the EasyAna dsRNA Quantitative Detection Kit (ELISA), designed for precise dsRNA measurement in virology, RNAi, and gene silencing studies.
Catalog-Number:
DC103
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Explore Vazyme's FastPure Bacteria DNA Isolation Mini Kit for swift, high-quality bacterial DNA extraction. Ideal for PCR, genotyping, and microbiome studies, ensuring efficient and reliable results. Perfect for rapid research needs.
Catalog-Number:
R433
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
1-2 weeks
The HiScript IV All-in-One Ultra RT SuperMix for qPCR from Vazyme is a next-generation reverse transcription kit specifically optimized for high-performance qPCR workflows. The innovative all-in-one formulation integrates genomic DNA removal and first-strand cDNA synthesis into a single-tube reaction, enabling rapid and highly efficient reverse transcription with minimal hands-on time.
Powered by the advanced HiScript IV Reverse Transcriptase, heat-labile DNase, and an optimized proprietary buffer system, the kit completes genomic DNA elimination and reverse transcription in as little as 5 minutes. The resulting cDNA is fully compatible with both SYBR dye-based and probe-based qPCR applications.
Key Features
All-in-one workflow: Combines genomic DNA removal and reverse transcription in a single reaction tube.
Ultra-fast protocol: Complete cDNA synthesis and gDNA elimination in only 5 minutes.
High reverse transcription efficiency: Produces higher cDNA yields and lower Ct values for accurate detection of low-expression targets.
Broad template compatibility: Suitable for animal, plant, and microbial RNA samples.
Compatible with dye- and probe-based qPCR: Flexible integration into various qPCR workflows.
Reduced contamination risk: Fewer pipetting steps improve reproducibility and workflow safety.
Excellent performance with difficult samples: Efficient reverse transcription of low-input and partially degraded RNA.
Ready-to-use premix: Contains reverse transcriptase, HL-DNase, RNase inhibitor, dNTPs, and optimized primer mix.
Applications
Reverse transcription for RT-qPCR
Gene expression analysis
Low-abundance transcript detection
RNA quantification studies
High-throughput qPCR workflows
Analysis of animal, plant, and microbial RNA
Validation of transcriptomics and RNA-seq data
Advantages
The highly optimized enzyme system significantly improves reverse transcription efficiency, generating high-quality cDNA suitable for sensitive downstream qPCR analysis. Lower Ct values compared to conventional reverse transcription kits enable improved detection of low-expression genes and challenging RNA templates.
The simplified one-tube workflow minimizes handling errors, reduces contamination risks, and accelerates laboratory workflows, making the kit ideal for routine molecular biology applications as well as high-throughput research environments.
Catalog-Number:
D101-01
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
MycoBlue Mycoplasma Detector: Your Solution for Reliable Mycoplasma Detection
Product Overview:The MycoBlue Mycoplasma Detector offers a straightforward and efficient method for detecting mycoplasma contamination in your cell cultures, ensuring the accuracy and reliability of your bioresearch. Designed for simplicity and efficacy, this detector is an essential tool for any life sciences laboratory.
Key Features:
Speed: Quickly identify mycoplasma contamination to save time and safeguard your experiments.
Accuracy: Benefit from high detection accuracy, minimizing the risk of false positives or negatives.
User-Friendly: Easy-to-use protocol that integrates seamlessly into your lab workflows.
Application Versatility: Suitable for a broad range of cell cultures, enhancing its utility in diverse research settings.
Why Choose MycoBlue Mycoplasma Detector?
When it comes to maintaining the integrity of your cell culture experiments, the MycoBlue Mycoplasma Detector provides peace of mind with its rapid and accurate testing process. Adopt it in your laboratory to ensure consistent and reliable results, contributing to the success and credibility of your research.
Catalog-Number:
D201
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
1-2 weeks
The Mycolor One-Step Mycoplasma Detector from Vazyme is a fast and highly sensitive solution for detecting mycoplasma contamination in cell cultures.
Based on advanced isothermal amplification technology, this assay enables reliable mycoplasma detection with a simple one-tube workflow and direct visual readout.
After adding only 1 μl of cell culture supernatant and incubating at 65°C for 1 hour, results can be interpreted by color change alone.
Negative samples remain purple-red, while positive samples turn yellow, providing a clear and intuitive result without gel electrophoresis or qPCR analysis.
Key Features
Rapid detection in approximately 1 hour
Simple one-tube workflow with minimal hands-on time
Visual colorimetric readout without specialized detection instruments
High sensitivity and specificity comparable to qPCR
Anti-contamination system reduces false-positive results
Compatible with adherent and suspension cell cultures
Broad compatibility with various media and serum types
Detects multiple mycoplasma species commonly found in cell culture
Applications
Routine monitoring of cell culture contamination
Quality control in cell biology laboratories
Screening of adherent and suspension cells
Research involving sensitive cell culture systems
Advantages
No PCR or qPCR instrument required
Easy interpretation through distinct color contrast
Reliable detection with built-in anti-aerosol contamination protection
Convenient workflow suitable for daily laboratory use
The Mycolor One-Step Mycoplasma Detector is an efficient and user-friendly tool for maintaining contamination-free cell cultures and ensuring reliable experimental results.
Catalog-Number:
B2311
Brand:
Selleck Chemicals LLC
Exp. delivery time:
1-2 weeks
Discover Poly FLAG Peptide Lyophilized Powder for your research: high-purity, stable, and versatile for protein purification and detection. Ideal for molecular biology applications.
Catalog-Number:
N409
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Improve plant RNA-seq quality with Ribo-off rRNA Depletion Kit (Plant). Efficiently remove rRNA, enrich mRNA for deeper transcriptomics analysis.
Catalog-Number:
N417
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Advance your bacterial RNA-seq with the Ribo-off rRNA Depletion Kit V2, designed for precise rRNA removal from bacterial samples, enabling enhanced gene expression analysis.
Catalog-Number:
Q713
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
1-2 weeks
The SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+) from Vazyme is a premium dye-based real-time PCR master mix specifically optimized for highly sensitive and precise quantitative PCR applications. Utilizing an advanced SYBR Green I fluorescence system, the kit delivers exceptional amplification specificity and stability, even for low-abundance targets and challenging sample types.
Powered by a BioSmart platform-selected hot-start Taq polymerase with enhanced 3′-end mismatch recognition, the master mix ensures superior amplification accuracy and minimal non-specific amplification. The optimized buffer system combined with dual-species antibody hot-start technology enables robust amplification performance across a broad range of templates, including degraded samples and GC-rich targets.
Key Features
High sensitivity for low-expression genes: Reliable detection of low-template and low-abundance targets.
Excellent specificity: Advanced hot-start technology minimizes non-specific amplification and primer-dimer formation.
Complex template compatibility: Efficient amplification of degraded RNA-derived cDNA and GC-rich templates.
dUTP/Heat-labile UDG anti-contamination system: Prevents carryover contamination and improves qPCR reliability.
Universal ROX compatibility: Includes optimized ROX passive reference dye compatible with multiple qPCR instruments without adjustment.
Premixed convenience: Ready-to-use formulation reduces pipetting steps and improves reproducibility.
Stable performance: Maintains reliable amplification performance even after prolonged premixed storage.
Easy sample handling: Purple-colored master mix facilitates accurate pipetting and loading.
Applications
Gene expression analysis
Real-time quantitative PCR (RT-qPCR)
Low-abundance transcript detection
Absolute and relative quantification
Validation of RNA-seq results
Detection of difficult or degraded samples
High-throughput qPCR workflows
Advantages
The optimized enzyme and buffer system provide excellent amplification efficiency across a wide linear range while maintaining high sensitivity and specificity. The integrated contamination prevention system effectively removes contaminating uracil-containing DNA at room temperature, helping ensure highly reliable qPCR results.
The master mix is compatible with a wide variety of templates including genomic DNA, cDNA, plasmid DNA, and λDNA, making it suitable for both routine and advanced gene expression studies.
Catalog-Number:
TR101
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Enhance your RNA research with the T7 High Yield RNA Transcription Kit. Achieve top-tier yield and purity across various applications, from RNA probes to therapeutics. Reliable, efficient, and designed for ease of use.
Catalog-Number:
TR102
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Explore our T7 RNAi Transcription Kit for efficient RNA interference studies. High-purity RNA synthesis, versatile applications, and user-friendly components. Ideal for advanced gene research.
Catalog-Number:
N411
Brand:
Nanjing Vazyme Biotech Co., Ltd.
Exp. delivery time:
3-4 weeks
Optimize your lab workflow with VAHTS DNA Clean Beads, ideal for high-quality DNA purification across various applications like PCR, sequencing, and cloning.