2 × Rapid Taq Plus Master Mix (Dye Plus)

The 2× Rapid Taq Plus Master Mix (Dye Plus) from Vazyme is a high-performance ready-to-use PCR amplification solution designed for fast, reliable, and highly reproducible DNA amplification. The optimized premixed formulation contains Rapid Taq Plus DNA Polymerase, dNTPs, Mg²⁺, reaction buffer, stabilizers, and loading dye, significantly reducing pipetting steps and minimizing experimental variation.

Featuring advanced hot-start technology with dual-blocking antibodies and an optimized amplification system, this master mix delivers excellent specificity, sensitivity, and amplification success rates across a broad range of templates and GC contents.

Key Features

• Ultra-fast PCR amplification: Extension speed as fast as 1–10 sec/kb, greatly reducing PCR runtime.
• Ready-to-use formulation: Simply add primers and template DNA.
• Hot-start technology: Dual-antibody blocking system improves specificity and sensitivity.
• Broad template compatibility: Efficient amplification of genomic DNA, plasmid DNA, λDNA, and cDNA templates.
• Supports long fragment amplification: Up to 15 kb genomic/plasmid targets and up to 10 kb cDNA targets.
• Wide GC compatibility: Robust performance across low and high GC-content templates.
• Direct gel loading: Integrated tracking dye allows direct electrophoresis after PCR.
• Freeze-thaw stability: Special stabilizers prevent freezing at -20°C and maintain enzyme activity.

Applications

• Rapid PCR amplification
• Routine molecular cloning
• Colony PCR
• Genotyping
• Template screening
• DNA fragment amplification
• High-throughput PCR workflows

Advantages

The optimized reaction chemistry provides high amplification efficiency and strong specificity while reducing overall workflow time. The included loading dye eliminates the need for additional sample preparation before agarose gel electrophoresis, streamlining daily PCR applications.

PCR products generated with this mix contain 3′-A overhangs, making them compatible with TA cloning workflows and Vazyme cloning systems such as ClonExpress and TOPO cloning kits.