2 × Phanta Ultra Master Mix

The 2× Phanta Ultra Master Mix from Vazyme is a next-generation high-fidelity PCR master mix developed for ultra-accurate, rapid, and highly efficient DNA amplification. Powered by the advanced Phanta Ultra DNA Polymerase and optimized through the BioSmart platform, this master mix combines exceptional fidelity with fast extension rates and broad compatibility across diverse PCR applications.

The optimized hot-start system and superior reaction buffer provide outstanding amplification specificity and high success rates, even for challenging templates such as GC-rich, AT-rich, crude, or multiplex PCR systems. With a fidelity approximately 200× higher than conventional Taq polymerase, the kit is ideal for applications requiring highly precise amplification.

Key Features

• Ultra-high fidelity: Approximately 200× higher fidelity than standard Taq DNA polymerase.
• Rapid amplification: Extension speed of 5 sec/kb for fragments under 10 kb.
• Universal annealing conditions: Broad Tm compatibility enables simplified PCR setup.
• Hot-start technology: Improved specificity and reduced non-specific amplification.
• Long fragment amplification: Supports amplification of fragments up to 40 kb.
• Broad template compatibility: Suitable for genomic DNA, plasmids, cDNA, crude samples, GC-rich and AT-rich templates.
• Ready-to-use format: Contains DNA polymerase, dNTPs, Mg²⁺, and optimized buffer.
• Blunt-end PCR products: Compatible with downstream cloning workflows such as ClonExpress and TOPO cloning.

Applications

• High-fidelity PCR amplification
• Molecular cloning
• DNA sequencing preparation
• Site-directed mutagenesis
• Long-range PCR
• Multiplex PCR
• GC-rich template amplification
• Gene synthesis and verification

Advantages

The optimized reaction chemistry enables reliable amplification with minimal optimization, making the workflow highly efficient for routine and advanced molecular biology applications. The broad Tm compatibility allows multiple targets with varying primer melting temperatures to be amplified simultaneously using a unified PCR protocol.

Its combination of ultra-high fidelity, rapid cycling, and excellent robustness makes the 2× Phanta Ultra Master Mix an excellent choice for research applications where precision and speed are critical.