HiScript IV All-in-One Ultra RT SuperMix for qPCR

The HiScript IV All-in-One Ultra RT SuperMix for qPCR from Vazyme is a next-generation reverse transcription kit specifically optimized for high-performance qPCR workflows. The innovative all-in-one formulation integrates genomic DNA removal and first-strand cDNA synthesis into a single-tube reaction, enabling rapid and highly efficient reverse transcription with minimal hands-on time.

Powered by the advanced HiScript IV Reverse Transcriptase, heat-labile DNase, and an optimized proprietary buffer system, the kit completes genomic DNA elimination and reverse transcription in as little as 5 minutes. The resulting cDNA is fully compatible with both SYBR dye-based and probe-based qPCR applications.

Key Features

• All-in-one workflow: Combines genomic DNA removal and reverse transcription in a single reaction tube.
• Ultra-fast protocol: Complete cDNA synthesis and gDNA elimination in only 5 minutes.
• High reverse transcription efficiency: Produces higher cDNA yields and lower Ct values for accurate detection of low-expression targets.
• Broad template compatibility: Suitable for animal, plant, and microbial RNA samples.
• Compatible with dye- and probe-based qPCR: Flexible integration into various qPCR workflows.
• Reduced contamination risk: Fewer pipetting steps improve reproducibility and workflow safety.
• Excellent performance with difficult samples: Efficient reverse transcription of low-input and partially degraded RNA.
• Ready-to-use premix: Contains reverse transcriptase, HL-DNase, RNase inhibitor, dNTPs, and optimized primer mix.

Applications

• Reverse transcription for RT-qPCR
• Gene expression analysis
• Low-abundance transcript detection
• RNA quantification studies
• High-throughput qPCR workflows
• Analysis of animal, plant, and microbial RNA
• Validation of transcriptomics and RNA-seq data

Advantages

The highly optimized enzyme system significantly improves reverse transcription efficiency, generating high-quality cDNA suitable for sensitive downstream qPCR analysis. Lower Ct values compared to conventional reverse transcription kits enable improved detection of low-expression genes and challenging RNA templates.

The simplified one-tube workflow minimizes handling errors, reduces contamination risks, and accelerates laboratory workflows, making the kit ideal for routine molecular biology applications as well as high-throughput research environments.