SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+)

The SupRealQ Ultra Hunter SYBR qPCR Master Mix (U+) from Vazyme is a premium dye-based real-time PCR master mix specifically optimized for highly sensitive and precise quantitative PCR applications. Utilizing an advanced SYBR Green I fluorescence system, the kit delivers exceptional amplification specificity and stability, even for low-abundance targets and challenging sample types.

Powered by a BioSmart platform-selected hot-start Taq polymerase with enhanced 3′-end mismatch recognition, the master mix ensures superior amplification accuracy and minimal non-specific amplification. The optimized buffer system combined with dual-species antibody hot-start technology enables robust amplification performance across a broad range of templates, including degraded samples and GC-rich targets.

Key Features

• High sensitivity for low-expression genes: Reliable detection of low-template and low-abundance targets.
• Excellent specificity: Advanced hot-start technology minimizes non-specific amplification and primer-dimer formation.
• Complex template compatibility: Efficient amplification of degraded RNA-derived cDNA and GC-rich templates.
• dUTP/Heat-labile UDG anti-contamination system: Prevents carryover contamination and improves qPCR reliability.
• Universal ROX compatibility: Includes optimized ROX passive reference dye compatible with multiple qPCR instruments without adjustment.
• Premixed convenience: Ready-to-use formulation reduces pipetting steps and improves reproducibility.
• Stable performance: Maintains reliable amplification performance even after prolonged premixed storage.
• Easy sample handling: Purple-colored master mix facilitates accurate pipetting and loading.

Applications

• Gene expression analysis
• Real-time quantitative PCR (RT-qPCR)
• Low-abundance transcript detection
• Absolute and relative quantification
• Validation of RNA-seq results
• Detection of difficult or degraded samples
• High-throughput qPCR workflows

Advantages

The optimized enzyme and buffer system provide excellent amplification efficiency across a wide linear range while maintaining high sensitivity and specificity. The integrated contamination prevention system effectively removes contaminating uracil-containing DNA at room temperature, helping ensure highly reliable qPCR results.

The master mix is compatible with a wide variety of templates including genomic DNA, cDNA, plasmid DNA, and λDNA, making it suitable for both routine and advanced gene expression studies.