2 × Phanta Ultra Master Mix

The 2 × Phanta Ultra Master Mix from Vazyme is a ready-to-use high-fidelity PCR master mix designed for fast, accurate and robust DNA amplification. It is based on Phanta Ultra DNA Polymerase and is suitable for demanding molecular biology workflows such as molecular cloning, gene identification, gene synthesis and site-directed mutagenesis.

Product overview

This 2× master mix contains the essential PCR components required for amplification, including Phanta Ultra DNA Polymerase, dNTPs, Mg²⁺ and an optimized reaction buffer. Only primers, template DNA and nuclease-free water need to be added, helping to reduce pipetting steps and improve reproducibility.

Key features

• High-fidelity PCR master mix for accurate DNA amplification
• Approximately 200× higher fidelity than standard Taq DNA polymerase
• Fast extension speed: 5 sec/kb for fragments below 10 kb and 10 sec/kb for fragments of 10 kb or longer
• Universal annealing program with primer annealing at 60°C
• Broad template compatibility, including complex templates and GC-rich or AT-rich systems
• Suitable for multiplex amplification workflows
• Can amplify long fragments up to 40 kb, depending on template quality and reaction conditions
• Produces blunt-ended PCR products suitable for downstream cloning workflows

Applications

• High-fidelity PCR
• Molecular cloning
• Gene identification
• Gene synthesis
• Site-directed mutagenesis
• Long-fragment PCR
• Multiplex PCR
• Amplification of GC-rich, AT-rich or complex DNA templates