2 × Phanta Ultra Master Mix (Dye Plus)

The 2 × Phanta Ultra Master Mix (Dye Plus) from Vazyme is a ready-to-use high-fidelity PCR master mix designed for fast, accurate and robust DNA amplification. This Dye Plus version contains an electrophoresis indicator dye, allowing PCR products to be loaded directly onto an agarose gel after amplification.

Product overview

The master mix is based on Phanta Ultra DNA Polymerase and contains the essential PCR reaction components, including DNA polymerase, dNTPs, Mg²⁺, optimized PCR buffer and loading dye. Only primers, template DNA and nuclease-free water need to be added, reducing pipetting steps and improving workflow consistency.

Key features

• High-fidelity PCR master mix for accurate DNA amplification
• Dye Plus format with electrophoresis/loading dye included
• Allows direct agarose gel loading after PCR
• Approximately 200× higher fidelity than standard Taq DNA polymerase
• Fast extension speed for rapid PCR workflows
• Universal annealing program with broad primer Tm compatibility
• Suitable for complex templates, including GC-rich and AT-rich systems
• Can amplify long fragments depending on template quality and reaction conditions
• Produces blunt-ended PCR products suitable for downstream cloning workflows

Applications

• High-fidelity PCR
• DNA amplification with direct gel loading
• Molecular cloning
• Gene identification
• Gene synthesis
• Site-directed mutagenesis
• Long-fragment PCR
• Multiplex PCR
• Amplification of GC-rich, AT-rich or complex DNA templates