2 × KeyPo SE Master Mix (Dye Plus)

Overview

2 × KeyPo SE Master Mix (Dye Plus) is a research-use mix supporting high-fidelity PCR workflows. It is applicable for regular templates, crude samples, uracil-containing DNA, long fragments (40 kb), and GC-rich systems (primers or templates). The wording is kept close to the supplied product information, helping you reference the right item quickly and document consistently.

Key Benefits

• Long-range amplification: gDNA≤40 kb, Plasmid≤30 kb, λDNA≤17.8 kb and cDNA≤.
• Compatible with 12-plex PCR of 300 bp-15 kb fragmentsHigh success rate: Robust amplification of complex systems (GC-rich/AT-rich, lysis products, plaque/bacterial suspension, whole blood/cell/leaf direct amplification, etc.
• It is applicable for regular templates, crude samples, uracil-containing DNA, long fragments (40 kb), and GC-rich systems (primers or templates).
• The amplification products are blunt-ended, which are subsequently applicable for the ClonExpress Ultra One Step Cloning Kit V2 ( #C116) and the Ultra-Universal TOPO Cloning Kit.

How it supports your workflow

In day-to-day research, teams often need products that fit existing methods while remaining easy to justify in notes, SOPs, and reporting. For high-fidelity PCR workflows, the goal is often to keep the scientific rationale and the purchasing record aligned. With the latest hot start technology and optimized buffer system, it can perform highly specific single-plex PCR for up to 40 kb, and highly uniform multiplex PCR within the range of 300 bp - 15 kb, which minimizes GC bias and allows for more uniform. It is applicable for regular templates, crude samples, uracil-containing DNA, long fragments (40 kb), and GC-rich systems (primers or templates). The amplification products are blunt-ended, which are subsequently applicable for the ClonExpress Ultra One Step Cloning Kit V2 ( #C116) and the Ultra-Universal TOPO Cloning Kit ( #C603). The supplied feature set highlights long-range amplification and compatible with 12-plex pcr of 300 bp-15 kb fragmentshigh success rate, which can help teams standardize language and expectations across similar products. Because projects evolve, we aim for descriptions that remain searchable, specific, and easy to compare across catalog numbers—so you can reorder confidently and keep documentation consistent.

Applications & compatibility

Use the notes below to gauge fit for your setup and documentation needs. With the latest hot start technology and optimized buffer system, it can perform highly specific single-plex PCR for up to 40 kb, and highly uniform multiplex PCR within the range of 300 bp - 15. If you’re matching this item to an established SOP, use the product name, catalog number, and the supplied features as the primary reference points. Use the application notes as a scope guide: they indicate fit and compatibility language drawn from the supplied product information, not assumptions beyond it.

Ideal for: labs focused on high-fidelity PCR workflows; teams that need clear, searchable documentation for purchasing and experimental records; and groups standardizing methods across projects or sites. Ideal when you need a quick, confident match between what’s on the page and what’s in your records.