Anoctamin-1 (ANO1 or TMEM16A) (C-Terminus) Antibody - Western Blot Validated
BiCell Scientific
SKU:20530-WB-50UL
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Protein Function
Bring Anoctamin-1 C-Terminus (ANO1) into view to analyze changes across conditions. Anoctamin-1 C-Terminus is a trafficking-sensitive transporter that can shift between intracellular pools and the plasma membrane. Focus on trends across conditions rather than single-point snapshots.
Tracking Anoctamin-1 C-Terminus can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Experimental Applications
Transport targets are commonly studied under stimulation conditions that alter membrane potential, osmolarity, or nutrient availability. Pair measurements with orthogonal readouts when interpretation is sensitive to context.
Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.
Common experimental questions that benefit from target tracking include:
- relative abundance across engineered constructs
- surface versus intracellular pool distribution
- stimulus-dependent trafficking and turnover
- patterns consistent with apical or basolateral sorting
Workflow Perspective
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Anoctamin-1 C-Terminus patterns can be compared across experiments without guesswork.
For challenging targets, pairing Anoctamin-1 C-Terminus measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Choose the denaturing-format option when you want Anoctamin-1 C-Terminus (ANO1) results aligned to your sample format.
- Targets:
- ANO1
- Research Area:
- Metabolism
- Application:
- WB
- Reactivity:
- Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (14-aa) derived from the C-terminal cytoplasmic region of mouse Anoctamin-1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q5XXA6
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 64.3% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C