ARL13B (Human) (ARL2L1) HiRe Antibody - FFPE Validated

Biological Background

This antibody supports experimental designs that examine ARL13B with interpretable controls and clear contrasts. ARL13B is a trafficking-related component that can localize to basal bodies and ciliary axonemes. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol ARL2L1.

Use in the Laboratory

Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Time course design can be decisive when regulation is transient or compartment dependent.

Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.

To keep datasets interpretable, labs often document:

• changes in cilia incidence or apparent length in perturbation studies
• cell-cycle linked differences in localization
• co-occurrence with intraflagellar transport markers

Version Notes

This fixed-section oriented version is intended for paraffin-embedded tissue, where crosslinking and retrieval steps can shape epitope accessibility. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Predefine decision rules for excluding outliers so analysis remains transparent.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so ARL13B patterns can be compared across experiments without guesswork.

Use the fixed-section option to keep ARL13B analysis aligned with planned contrasts and endpoints.