BCL2 Antibody - Western Blot Validated
BiCell Scientific
SKU:10250-WB-50UL
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About the Target
For consistent comparison across experiments, use this antibody to explore BCL2 with a repeatable workflow. BCL2 is a cellular protein that can be tracked to understand expression patterns and compartmental behavior. Use matched handling so differences reflect biology rather than processing.
Monitoring BCL2 alongside context markers can improve interpretation when phenotypes are subtle.
Research Context
Temporal sampling and matched controls can improve interpretation when expression is dynamic. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Consider these angles when interpreting target-level changes:
- subcellular compartment mapping
- baseline versus stimulated condition comparisons
- cross-sample normalization strategies
- interpretation alongside orthogonal readouts
Variant Considerations
When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. This matters because epitope exposure and background can vary with preparation and extraction steps. Standardize key parameters so differences reflect biology rather than handling.
If your study spans multiple model systems, it can help to define a shared reference condition for BCL2 so cross-sample contrasts stay meaningful.
If your study spans multiple model systems, it can help to define a shared reference condition for BCL2 so cross-sample contrasts stay meaningful.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so BCL2 patterns can be compared across experiments without guesswork.
With consistent handling, the denaturing-format format can make BCL2 comparisons easier to reproduce across runs.
- Targets:
- BCL2
- Research Area:
- Apoptosis • Cancer • Metabolism
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the N-terminal region of mouse BCL2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P10415
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 94.1% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C
For Research Use Only. Not intended for diagnostic or therapeutic use.
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The purchase of this product does not grant any license for commercial use, manufacturing, or clinical applications. The user is responsible for ensuring compliance with applicable laws and third-party rights.