CCR2 (CD192) Antibody - Western Blot Validated

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BiCell Scientific

SKU:64132-WB-50UL

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Size
Blocking Peptide
Quantity
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About the Target

Build reproducible readouts by using this antibody to assess CCR2 across matched sample sets. CCR2 is a surface or signaling marker used to characterize immune cell subsets and activation states. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.

Monitoring CCR2 alongside context markers can improve interpretation when phenotypes are subtle.

Research Context

Immune markers are often interpreted alongside a panel of complementary targets to improve population resolution. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.

Consider these angles when interpreting target-level changes:

  • activation-dependent changes across stimulation conditions
  • panel-based interpretation with additional lineage markers
  • comparisons across differentiation or polarization states
  • assessment of surface versus internalized pools

Variant Considerations

This denaturing-format version is geared toward targets presented after reduction and denaturation, where epitope exposure can shift from native conformations. This matters because epitope exposure and background can vary with preparation and extraction steps. Standardize key parameters so differences reflect biology rather than handling.

When interpreting CCR2, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

For challenging targets, pairing CCR2 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

With consistent handling, the denaturing-format format can make CCR2 comparisons easier to reproduce across runs.

Targets:
CCR2
Research Area:
Cell Signaling • Developmental Biology • Immunology • Inflammation
Application:
WB
Reactivity:
Human • Mouse • Rat
Host:
Rat
Clonality:
Polyclonal
Isotype:
IgG
Immunogen:
Synthetic peptide (15-aa) derived from the C-terminal cytoplasmic region of mouse CCR2 protein
Conjugation:
Unconjugated
Purification:
Affinity Chromatography
Concentration:
0.25 mg/ml
UniProt:
P41597
Homology:
Synthetic peptide sequence is identical to rat sequence (showing 86.7% homology to human sequence)
Storage Buffer:
PBS, pH 7.2, 0.1% Sodium Azide
Storage Temperature:
-20°C