CD226 Antibody

About the Target

CD226 (DNAM-1) is a transmembrane glycoprotein expressed predominantly on immune cells like T cells, NK cells, and monocytes. It contains two immunoglobulin V-like domains (D1 and D2) in its extracellular region, a type I transmembrane domain, and an intracellular cytoplasmic domain with critical phosphorylation sites at Y322 and S329 in humans. Depending on the literature source, CD226 may also be discussed as DNAM-1.

Reported cellular context includes cell membrane and membrane, which can matter when signal is compared across treatments or changing cell states. Following CD226 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

CD226 is commonly interpreted in the context of cancer, immunology, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell membrane and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell membrane and membrane across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• context differences tied to immune-cell state, activation, or lineage composition
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for CD226. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in CD226 reflect biology rather than handling. When interpreting CD226, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep CD226 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.