CD45 (Intracellular Domain) Antibody

About the Target

CD45 (Intracellular Domain) is a target of interest in many antibody-based workflows. CD45, also known as the leukocyte common antigen is a type 1 glycoprotein and receptor-like protein tyrosine phosphatase (RPTP) encoded by Ptprc, is expressed on all nucleated hematopoietic cells and plays a critical role in T and B cell development and activation. It has a large extracellular domain, a single transmembrane domain, and a cytoplasmic portion that consists of a wedge-like structure followed by tandem PTP domains (D1 and D2), and a 79 amino acid C-terminal tail.

Reported cellular context includes cell membrane, membrane, and synapse, which can matter when signal is compared across treatments or changing cell states. Following CD45 (Intracellular Domain) across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

CD45 (Intracellular Domain) is commonly interpreted in the context of immunology and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell membrane, membrane, and synapse, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell membrane, membrane, and synapse across matched conditions
• context differences tied to immune-cell state, activation, or lineage composition
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for CD45 (Intracellular Domain). This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in CD45 (Intracellular Domain) reflect biology rather than handling. When interpreting CD45 (Intracellular Domain), it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep CD45 (Intracellular Domain) trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.