CDC16 Antibody - Western Blot Validated
BiCell Scientific
SKU:51356-WB-50UL
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About the Target
To connect mechanism with measurement, this antibody lets you characterize CDC16 in pathway-driven studies. CDC16 is a pathway component whose activation state and localization can change after stimulation. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.
Tracking CDC16 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Research Context
Perturbations such as inhibitors or ligand stimulation can drive rapid shifts in localization or complex formation. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution.
Consider these angles when interpreting target-level changes:
- time-resolved changes following stimulation or inhibition
- condition-to-condition comparisons with matched controls
- localization shifts between cytoplasm, membrane, and nucleus
Variant Considerations
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. This matters because epitope exposure and background can vary with preparation and extraction steps. If you compare conditions, keep processing steps uniform to support clean interpretation.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CDC16 patterns can be compared across experiments without guesswork.
When interpreting CDC16, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
Use the denaturing-format version to relate CDC16 behavior to upstream cues and downstream consequences.
- Targets:
- CDC16
- Research Area:
- Cell Cycle • Cell Signaling
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the C-terminal region of human CDC16 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q13042
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C