CDC42 Antibody
BiCell Scientific
SKU:00274-50UL
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Protein Function
This antibody is suited for researchers who want to assess CDC42 and relate it to cell-state biology. CDC42 is a scaffold that can coordinate signaling with cytoskeletal remodeling. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
Tracking CDC42 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Experimental Applications
Drug perturbations that affect polymerization or contractility can shift distribution and complex formation. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.
Common experimental questions that benefit from target tracking include:
- differences across cell density or substrate stiffness
- stress-fiber versus cortical distribution patterns
- remodeling after perturbation of polymerization or contractility
Workflow Perspective
If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CDC42 patterns can be compared across experiments without guesswork.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CDC42 patterns can be compared across experiments without guesswork.
Use the regular format variant when your project demands CDC42 readouts that respect preparation constraints.
- Targets:
- CDC42
- Research Area:
- Cell Cycle • Cell Signaling • Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the internal region of human CDC42 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P60953
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C