CELSR1 (CDHF9 or FMI2) Antibody - Western Blot Validated

Biological Background

This antibody supports experimental designs that examine CELSR1 (FMI2) with interpretable controls and clear contrasts. CELSR1 is a contact-site protein that shapes barrier properties and signaling at the cell surface. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol FMI2.

Use in the Laboratory

Junction proteins are frequently analyzed during differentiation, stress responses, and changes in cell polarity. Time course design can be decisive when regulation is transient or compartment dependent. Neural differentiation and synaptic organization projects may include this target as a contextual readout.

Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.

To keep datasets interpretable, labs often document:

• subcellular redistribution at contact sites versus cytoplasm
• changes in complex assembly with scaffold or adaptor proteins
• comparative profiling across cell states or perturbations

Version Notes

When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Predefine decision rules for excluding outliers so analysis remains transparent.

When interpreting CELSR1, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

Use the denaturing-format option to keep CELSR1 (FMI2) analysis aligned with planned contrasts and endpoints.