CELSR2 (CDHF10 or Flamingo1) Antibody

Biological Background

This antibody supports experimental designs that examine CELSR2 (CDHF10) with interpretable controls and clear contrasts. CELSR2 is a structural regulator of intercellular boundaries and tissue architecture. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol CDHF10.

Use in the Laboratory

Shifts in junction organization can accompany migration, tissue morphogenesis, and altered mechanical tension. Time course design can be decisive when regulation is transient or compartment dependent. Planar polarity studies may connect this target to directional signaling and cytoskeletal alignment.

Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.

To keep datasets interpretable, labs often document:

• comparative profiling across cell states or perturbations
• subcellular redistribution at contact sites versus cytoplasm
• changes in complex assembly with scaffold or adaptor proteins

Version Notes

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Predefine decision rules for excluding outliers so analysis remains transparent.

For challenging targets, pairing CELSR2 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

The regular format format fits designs where CELSR2 (CDHF10) is evaluated with deliberate controls and careful comparisons.