CEP104 Antibody - FFPE Validated

Biological Background

Build reproducible readouts by using this antibody to characterize CEP104 across matched sample sets. CEP104 is a ciliary or centrosomal protein connected to microtubule-based organization. Use matched handling so differences reflect biology rather than processing.

Following CEP104 across perturbations can help separate expression effects from compartment shifts.

Use in the Laboratory

Disruption of intraflagellar transport or basal body function can produce distinct localization signatures. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.

Depending on the model, researchers may ask whether signal shifts reflect altered abundance, compartment changes, or remodeling of associated complexes.

To keep datasets interpretable, labs often document:

• cell-cycle linked differences in localization
• changes in cilia incidence or apparent length in perturbation studies
• co-occurrence with intraflagellar transport markers
• signal enrichment at basal bodies versus cytoplasm

Version Notes

For paraffin-embedded samples, fixation history and retrieval strategy matter; this option is aligned to those constraints. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. A brief pilot run can help set exposure ranges and reduce reruns later.

For challenging targets, pairing CEP104 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

Pair this fixed-section format with stable controls to keep CEP104 measurements coherent over time.