CEP135 (KIAA0635) Antibody - Western Blot Validated

Biological Background

When you need to assess CEP135 with attention to cellular organization, this antibody provides a focused option. CEP135 is a trafficking-related component that can localize to basal bodies and ciliary axonemes. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.

In some datasets and protocols, it is referenced by the gene symbol KIAA0635.

Use in the Laboratory

Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.

Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.

To keep datasets interpretable, labs often document:

• signal enrichment at basal bodies versus cytoplasm
• changes in cilia incidence or apparent length in perturbation studies
• co-occurrence with intraflagellar transport markers

Version Notes

When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Small differences in preparation can reshape apparent localization, so interpret results in context.

For challenging targets, pairing CEP135 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

Use the denaturing-format variant when your project demands CEP135 readouts that respect preparation constraints.