CEP164 (NPHP15) Antibody - FFPE Validated

Protein Function

This antibody supports experimental designs that analyze CEP164 with interpretable controls and clear contrasts. CEP164 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

Following CEP164 across perturbations can help separate expression effects from compartment shifts.

Experimental Applications

Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Time course design can be decisive when regulation is transient or compartment dependent.

In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.

Common experimental questions that benefit from target tracking include:

• signal enrichment at basal bodies versus cytoplasm
• co-occurrence with intraflagellar transport markers
• cell-cycle linked differences in localization

Workflow Perspective

For paraffin-embedded samples, fixation history and retrieval strategy matter; this option is aligned to those constraints. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.

When interpreting CEP164, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

If your study spans multiple model systems, it can help to define a shared reference condition for CEP164 so cross-sample contrasts stay meaningful.

Use the fixed-section option to keep CEP164 analysis aligned with planned contrasts and endpoints.