CEP170 (KIAA0470) Antibody - Western Blot Validated

Protein Function

This antibody supports experimental designs that examine CEP170 with interpretable controls and clear contrasts. CEP170 is a structural factor that supports primary cilium assembly or signaling competence. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol KIAA0470.

Experimental Applications

Disruption of intraflagellar transport or basal body function can produce distinct localization signatures. Time course design can be decisive when regulation is transient or compartment dependent.

Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.

Common experimental questions that benefit from target tracking include:

• changes in cilia incidence or apparent length in perturbation studies
• co-occurrence with intraflagellar transport markers
• cell-cycle linked differences in localization

Workflow Perspective

For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.

When interpreting CEP170, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

Use the denaturing-format option to keep CEP170 analysis aligned with planned contrasts and endpoints.