CEP250 Antibody
BiCell Scientific
SKU:01095-50UL
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Biological Background
Use this antibody to explore CEP250 in your model system. CEP250 is a structural factor that supports primary cilium assembly or signaling competence. Focus on trends across conditions rather than single-point snapshots.
Tracking CEP250 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Use in the Laboratory
Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Pair measurements with orthogonal readouts when interpretation is sensitive to context.
Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.
To keep datasets interpretable, labs often document:
- changes in cilia incidence or apparent length in perturbation studies
- cell-cycle linked differences in localization
- co-occurrence with intraflagellar transport markers
- signal enrichment at basal bodies versus cytoplasm
Version Notes
If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.
For challenging targets, pairing CEP250 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
If your study spans multiple model systems, it can help to define a shared reference condition for CEP250 so cross-sample contrasts stay meaningful.
Select the regular format version to keep CEP250 interpretation aligned with your workflow goals.
- Targets:
- CEP250
- Research Area:
- Cell Cycle • Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the N-terminal region of mouse CEP250 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9BV73
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 87.5% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C