CEP85 (CCDC21) Antibody - Western Blot Validated

Biological Background

Use this antibody to analyze CEP85 when mapping complex behavior and signaling context. CEP85 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.

In some datasets and protocols, it is referenced by the gene symbol CCDC21.

Use in the Laboratory

Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution.

Many targets show context-dependent localization, so comparisons across matched conditions can reveal meaningful patterns.

To keep datasets interpretable, labs often document:

• signal enrichment at basal bodies versus cytoplasm
• cell-cycle linked differences in localization
• co-occurrence with intraflagellar transport markers

Version Notes

For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CEP85 patterns can be compared across experiments without guesswork.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CEP85 patterns can be compared across experiments without guesswork.

Choose the denaturing-format format to study CEP85 in a way that stays anchored to pathway logic.