CHRNA4 (NACHRA4) Antibody - Western Blot Validated
BiCell Scientific
SKU:15184-WB-50UL
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Protein Function
For consistent comparison across experiments, use this antibody to evaluate CHRNA4 with a repeatable workflow. CHRNA4 is a membrane protein linked to solute movement and gradient maintenance. Use matched handling so differences reflect biology rather than processing.
In some datasets and protocols, it is referenced by the gene symbol NACHRA4.
Experimental Applications
Many transport proteins cycle through endosomal pathways, making localization a key readout alongside abundance. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy. Neural differentiation and synaptic organization projects may include this target as a contextual readout.
Common experimental questions that benefit from target tracking include:
- patterns consistent with apical or basolateral sorting
- surface versus intracellular pool distribution
- stimulus-dependent trafficking and turnover
- relative abundance across engineered constructs
Workflow Perspective
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CHRNA4 patterns can be compared across experiments without guesswork.
For multi-run studies, the denaturing-format option helps keep CHRNA4 trends comparable from experiment to experiment.
- Targets:
- CHRNA4
- Research Area:
- Cell Signaling • Neuroscience
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (13-aa) derived from the N-terminal extracellular region of mouse CHRNA4 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P43681
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 92.3% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C