CXCR4 (CD184) Antibody - FFPE Validated

About the Target

This antibody supports experimental designs that evaluate CXCR4 with interpretable controls and clear contrasts. CXCR4 is a surface or signaling marker used to characterize immune cell subsets and activation states. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol CD184.

Research Context

Experimental perturbations such as cytokine exposure can reshape marker abundance and localization patterns. Time course design can be decisive when regulation is transient or compartment dependent.

Consider these angles when interpreting target-level changes:

• panel-based interpretation with additional lineage markers
• assessment of surface versus internalized pools
• activation-dependent changes across stimulation conditions

Variant Considerations

Because fixation can mask epitopes, fixed-section oriented formats aim to maintain recognition after standard deparaffinization and retrieval steps. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.

If your study spans multiple model systems, it can help to define a shared reference condition for CXCR4 so cross-sample contrasts stay meaningful.

For challenging targets, pairing CXCR4 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

Integrate the fixed-section version into your design so CXCR4 signals can be interpreted alongside matched controls.