Discover-sc WTA Kit V2

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Vazyme Biotech

N711-01

€869,00 EUR
€869,00 EUR
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The Discover-sc WTA Kit V2 from Vazyme is designed for whole transcriptome amplification from single cells and other low-input RNA samples. The kit enables first-strand cDNA synthesis and amplification from very small starting amounts, helping researchers generate sufficient cDNA for downstream sequencing and transcriptome analysis workflows.

Product overview

Discover-sc WTA Kit V2 uses Oligo dT priming for reverse transcription and the template-switching activity of Discover-sc Reverse Transcriptase to add an adapter sequence during cDNA synthesis. The resulting cDNA is then amplified by PCR to generate full-length cDNA amplification products. This workflow helps reduce 3' bias and rRNA contamination while supporting full transcriptome coverage.

Key features

  • Designed for single-cell and low-input whole transcriptome amplification
  • Suitable for 1–1,000 cells or 10 pg–10 ng total RNA input
  • Uses Oligo dT priming for poly(A)+ RNA-derived cDNA synthesis
  • Template-switching reverse transcription enables full-length cDNA amplification
  • Improved sensitivity for low-abundance genes and low-concentration templates
  • Helps reduce 3' end preference and rRNA contamination during cDNA synthesis
  • Amplified cDNA can be used for downstream sequencing library preparation workflows

Applications

  • Single-cell transcriptome sequencing workflows
  • Whole transcriptome amplification
  • Low-input RNA sequencing sample preparation
  • Gene expression analysis
  • Fusion transcript analysis
  • Alternative splicing analysis
  • Full-length cDNA amplification
Research Area:
Molecular Biology • Single-Cell Transcriptomics • RNA Sequencing • Gene Expression Analysis
Specificity:
Poly(A)+ transcripts from single cells or low-input total RNA
Storage Temperature:
-80°C • -20°C

For Research Use Only. Not intended for diagnostic or therapeutic use.
Products may be subject to intellectual property rights.

The purchase of this product does not grant any license for commercial use, manufacturing, or clinical applications. The user is responsible for ensuring compliance with applicable laws and third-party rights.