DLL1 (Delta1 or Delta) Antibody - Western Blot Validated
BiCell Scientific
SKU:51510-WB-50UL
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About the Target
Build reproducible readouts by using this antibody to investigate DLL1 across matched sample sets. DLL1 is a signaling node often evaluated by abundance, localization, and modification-dependent states. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
Monitoring DLL1 alongside context markers can improve interpretation when phenotypes are subtle.
Research Context
Perturbations such as inhibitors or ligand stimulation can drive rapid shifts in localization or complex formation. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Consider these angles when interpreting target-level changes:
- condition-to-condition comparisons with matched controls
- interaction patterns within multi-protein complexes
- localization shifts between cytoplasm, membrane, and nucleus
- time-resolved changes following stimulation or inhibition
Variant Considerations
This denaturing-format version is geared toward targets presented after reduction and denaturation, where epitope exposure can shift from native conformations. This matters because epitope exposure and background can vary with preparation and extraction steps. Standardize key parameters so differences reflect biology rather than handling.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so DLL1 patterns can be compared across experiments without guesswork.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so DLL1 patterns can be compared across experiments without guesswork.
With consistent handling, the denaturing-format format can make DLL1 comparisons easier to reproduce across runs.
- Targets:
- DLL1
- Research Area:
- Cell Signaling • Developmental Biology
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the C-terminal cytoplasmic region of mouse DLL1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- O00548
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 86.7% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C