DNMT3B Antibody - Western Blot Validated
BiCell Scientific
SKU:82024-WB-50UL
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Protein Function
Build reproducible readouts by using this antibody to explore DNMT3B across matched sample sets. DNMT3B is a cellular protein that can be tracked to understand expression patterns and compartmental behavior. Use matched handling so differences reflect biology rather than processing.
Tracking DNMT3B can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Experimental Applications
Integrating localization with abundance measurements can clarify whether changes reflect trafficking or regulation. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- cross-sample normalization strategies
- interpretation alongside orthogonal readouts
- subcellular compartment mapping
- baseline versus stimulated condition comparisons
Workflow Perspective
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
If your study spans multiple model systems, it can help to define a shared reference condition for DNMT3B so cross-sample contrasts stay meaningful.
When interpreting DNMT3B, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
With consistent handling, the denaturing-format format can make DNMT3B comparisons easier to reproduce across runs.
- Targets:
- DNMT3B
- Research Area:
- Epigenetics
- Application:
- WB
- Reactivity:
- Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the N-terminal region of mouse DNMT3B protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9UBC3
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 50% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C