ELISA "Do-it-yourself" Kit

The ELISA “Do-it-yourself” Kit provides a set of supplemental reagents and materials for developing and performing colorimetric ELISA workflows. The kit is designed for research-use assay development across common ELISA formats using an HRP/TMB-based signal detection system.

Key features

• Development kit for colorimetric ELISA workflows
• Supports direct, indirect, sandwich and competitive ELISA assay formats
• Uses an HRP/TMB-based detection system
• Includes high-binding 96-well strip microplates with 12 breakable strips of 8 wells
• Includes commonly required ELISA buffers, diluents, substrate, stop solution and plate covers
• Contains sufficient materials for five 96-well plate assays

Kit contents

• Clear 96-well strip microplate, EC-003-05: 5 plates
• 1x Coating Buffer, EL-C9-50ML: 150 ml
• 1x Stabilizer and Blocking Solution, EL-C1-250ML: 300 ml
• 10x Wash Buffer, EL-C2-250ML: 250 ml
• 1x Assay Diluent, EL-C3-250ML: 250 ml
• 1x Detection Diluent, EL-C4-250ML: 250 ml
• 1x One-step TMB Substrate: 60 ml
• 1x Stop Solution, EL-C8-100ML: 100 ml
• Adhesive Plate Covers: 15 units

Applications

• ELISA assay development
• Direct ELISA workflows
• Indirect ELISA workflows
• Sandwich ELISA workflows
• Competitive ELISA workflows
• Research-use protein and biomarker detection assay setup

Use in ELISA development

The kit provides general ELISA reagents and consumables for assay development. Users must still select the appropriate assay format and provide target-specific components such as capture antibodies, detection antibodies, antigens, standards or conjugates as required by the chosen ELISA design.