FAT4 (FAT-J or CDHF14) Antibody - FFPE Validated

Biological Background

To connect mechanism with measurement, this antibody lets you analyze FAT4 in pathway-driven studies. FAT4 is a membrane-associated component that helps organize cell-cell contacts and polarity. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.

In some datasets and protocols, it is referenced by the gene symbol FAT-J.

Use in the Laboratory

In cultured models, junctional readouts can change rapidly after calcium modulation or cytoskeletal perturbation. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution. Planar polarity studies may connect this target to directional signaling and cytoskeletal alignment.

Depending on the model, researchers may ask whether signal shifts reflect altered abundance, compartment changes, or remodeling of associated complexes.

To keep datasets interpretable, labs often document:

• comparative profiling across cell states or perturbations
• changes in complex assembly with scaffold or adaptor proteins
• subcellular redistribution at contact sites versus cytoplasm

Version Notes

For paraffin-embedded samples, fixation history and retrieval strategy matter; this option is aligned to those constraints. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding.

When interpreting FAT4, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.

Choose the fixed-section format to study FAT4 in a way that stays anchored to pathway logic.