FGFR3 (CD333) Antibody
BiCell Scientific
SKU:52013-50UL
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Biological Background
For consistent comparison across experiments, use this antibody to evaluate FGFR3 with a repeatable workflow. FGFR3 is a signaling node often evaluated by abundance, localization, and modification-dependent states. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
In some datasets and protocols, it is referenced by the gene symbol CD333.
Use in the Laboratory
Parallel measurement of upstream inputs and downstream readouts can sharpen interpretation of pathway behavior. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.
To keep datasets interpretable, labs often document:
- interaction patterns within multi-protein complexes
- condition-to-condition comparisons with matched controls
- time-resolved changes following stimulation or inhibition
- localization shifts between cytoplasm, membrane, and nucleus
Version Notes
This regular format is a practical starting point when you want flexibility across sample types and iterative optimization. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. A brief pilot run can help set exposure ranges and reduce reruns later.
If your study spans multiple model systems, it can help to define a shared reference condition for FGFR3 so cross-sample contrasts stay meaningful.
For multi-run studies, the regular format option helps keep FGFR3 trends comparable from experiment to experiment.
- Targets:
- FGFR3
- Research Area:
- Cell Signaling • Developmental Biology • Immunology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the C-terminal region of mouse FGFR3 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P22607
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 80% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C