Formin-1 (FMN1) Antibody
BiCell Scientific
SKU:30055-50UL
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Protein Function
This antibody supports experimental designs that explore Formin-1 with interpretable controls and clear contrasts. Formin-1 is a scaffold that can coordinate signaling with cytoskeletal remodeling. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
In some datasets and protocols, it is referenced by the gene symbol FMN1.
Experimental Applications
Comparative analysis across confluence states can reveal junctional coupling or stress-fiber reorganization. Time course design can be decisive when regulation is transient or compartment dependent.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- remodeling after perturbation of polymerization or contractility
- association with junctional complexes or focal adhesions
- differences across cell density or substrate stiffness
Workflow Perspective
If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Formin-1 patterns can be compared across experiments without guesswork.
The regular format format fits designs where Formin-1 is evaluated with deliberate controls and careful comparisons.
- Targets:
- FMN1
- Research Area:
- Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the C-terminal region of mouse formin-1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q68DA7
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 93.8% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C