GFAP Antibody - Western Blot Validated
BiCell Scientific
SKU:02405-WB-50UL
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Scientific Overview
Build reproducible readouts by using this antibody to investigate GFAP across matched sample sets. GFAP is a target that supports characterization of cellular state through expression and localization readouts. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
Tracking GFAP can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Research Relevance
Temporal sampling and matched controls can improve interpretation when expression is dynamic. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Because cellular networks are interconnected, changes in this target may coincide with shifts in related pathways, trafficking behavior, or structural remodeling. Neural differentiation and synaptic organization projects may include this target as a contextual readout.
When building a narrative around the target, it helps to track:
- interpretation alongside orthogonal readouts
- subcellular compartment mapping
- cross-sample normalization strategies
- baseline versus stimulated condition comparisons
Application-Specific Context
If your primary readout depends on denatured protein presentation, this option can streamline comparison across lanes and conditions. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. A short pilot series can help set exposure ranges and reduce reruns.
For challenging targets, pairing GFAP measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
With consistent handling, the denaturing-format format can make GFAP comparisons easier to reproduce across runs.
- Targets:
- GFAP
- Research Area:
- Neuroscience
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the C-terminal region of human GFAP protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- P14136
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C